黑色素瘤细胞株培养条件：

WANQUANPEIYANGJI： DMEMGAOTANG 10%TAINIUXUEQING

PEIYANGTIAOJIAN：37.0C  carbon dioxide（CO2）,5%

黑色素瘤细胞株传代方法：

SHOUDAOXIBAOHOU，QUCHUPEIYANGPINGZAIDAOZHIXIANWEIJINGXIAGUANCHAXIBAOSHENGZHANGQINGKUANG。

（YI）RUGUOXIBAOWEIZHANGMAN，YONG75%JIUJINGPENSAZHENGGEPINGXIAODUHOUFANGDAOCHAOJUNTAINEI，YANGEWUJUNCAOZUO，DAKAIXIBAOPEIYANGPING，XICHUPEIYANGYE，JINLIUXIA10mlPEIYANGYEZAIPINGNEIJIXUPEIYANG。

（ER）RUGUOXIBAOYIZHANGMAN，JIKEJINXINGCHUANDAIPEIYANG。JUTIBUZHOURUXIA：

1. QIQUPEIYANGYE，YONGPBS（BUHANGAI，MEILIZI）XI1-2CI。

2. JIA1mlXIAOHUAYE（0.25%Trypsin-0.53mM EDTA）YUPEIYANGPINGZHONG，DAOZHUANFANGYU37DUPEIYANGXIANG1-3FENZHONGYURE，RANHOUYOUJIANGPEIYANGPINGDAOZHUANDAYUE30MIAOHOU，ZAIDAOZHIXIANWEIJINGXIAGUANCHAXIBAOXIAOHUAQINGKUANG，RUOXIBAODABUFENBIANYUANFENSAN，QINGQIAOJIXIAPEIYANGPEIYANGPING，XIBAOSUIJITUOLUOXIALAI。

3. JIARU6-8mlWANQUANPEIYANGJI，XICHU，FENDAOXINDEPEIYANGPINGZHONG。YICHUANER。

ZHUYI：CHUANDAIHOUYIBANYONGWOMENDEPEIYANGJI，YIBANYONGNIMENDE，YIMIANXIBAOBUSHIYINGERZAO

CHENGSHENGZHANGBUHAO。

DONGCUNFANGFA：DONGCUNYE：95%WANQUANPEIYANGYE，5%DMSO

CHUCUN：YEDANCHUCUN

来自ATCC细胞，所有出入库细胞均经过严格的细胞质量检测，确保细胞无污染，符合检测合格标准。欢迎来询价详谈。